Journal: Frontiers in Nutrition

Article Title: The anti-allergic effect of Lactobacillus plantarum GUANKE on allergic rhinitis induced by either ragweed pollen or house dust mites in mice

doi: 10.3389/fnut.2025.1678249

Figure Lengend Snippet: The effect of GUANKE strain on AR symptoms induced by either RAGW or HDM in mice. From day 21 to day 49, the nose-wiping frequency, sneezing frequency, and their combined frequency in the RAGW-induced AR model (A) or HDM-induced AR model (C) ( n = 10). The “*” or “ns” in A,C represents the p -value of the AR group compared to the GK-H group. From day 21 to day 49, the AUC of nose-wiping frequency, sneezing frequency, and their combined frequency in the RAGW-induced AR model (B) or HDM-induced AR model (D) ( n = 10). The data are presented as the mean ± SEMs. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. RAGW, ragweed pollen; HDM, house dust mite; Ctrl, control; AR, allergic rhinitis; Ceti, Cetirizine; GK-L, low-dose GUANKE (10 6 CFU); GK-M, middle-dose GUANKE (10 8 CFU); GK-H, high-dose GUANKE (10 10 CFU).

Article Snippet: Cetirizine (HY-17042, MCE) was used as a positive control for this study.

Techniques: Control

Journal: Frontiers in Nutrition

Article Title: The anti-allergic effect of Lactobacillus plantarum GUANKE on allergic rhinitis induced by either ragweed pollen or house dust mites in mice

doi: 10.3389/fnut.2025.1678249

Figure Lengend Snippet: The effect of GUANKE strain on IgE in the serum of AR mice induced by either RAGW or HDM. (A) The total IgE levels in serum of each group of mice in RAGW-induced AR model ( n = 10). (B) The HDM-specific IgE levels in serum of each group of mice in HDM-induced AR model ( n = 10). The data are presented as the mean ± SEMs. * p < 0.05; ** p < 0.01; *** p < 0.001. RAGW, ragweed pollen; HDM, house dust mite; Ctrl, control; AR, allergic rhinitis; Ceti, Cetirizine; GK-L, low-dose GUANKE (10 6 CFU); GK-M, middle-dose GUANKE (10 8 CFU); GK-H, high-dose GUANKE (10 10 CFU).

Article Snippet: Cetirizine (HY-17042, MCE) was used as a positive control for this study.

Techniques: Control

Journal: Frontiers in Nutrition

Article Title: The anti-allergic effect of Lactobacillus plantarum GUANKE on allergic rhinitis induced by either ragweed pollen or house dust mites in mice

doi: 10.3389/fnut.2025.1678249

Figure Lengend Snippet: The effect of GUANKE strain on the levels of Th2 cytokines in the serum and nasal mucosa of AR mice induced by either RAGW or HDM. The levels of Th2 cytokines in the serum in the RAGW-induced AR model (A) and HDM-induced AR model (B) ( n = 10). The levels of Th2 cytokines in the nasal mucosa in the RAGW-induced AR model (C) and HDM-induced AR model (D) ( n = 5). The data are presented as the means ± SEMs. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. RAGW, ragweed pollen; HDM, house dust mite; Ctrl, control; AR, allergic rhinitis; Ceti, Cetirizine; GK-L, low-dose GUANKE (10 6 CFU); GK-M, middle-dose GUANKE (10 8 CFU); GK-H, high-dose GUANKE (10 10 CFU).

Article Snippet: Cetirizine (HY-17042, MCE) was used as a positive control for this study.

Techniques: Control

Journal: Frontiers in Nutrition

Article Title: The anti-allergic effect of Lactobacillus plantarum GUANKE on allergic rhinitis induced by either ragweed pollen or house dust mites in mice

doi: 10.3389/fnut.2025.1678249

Figure Lengend Snippet: The effect of GUANKE strain on histopathological changes in nasal mucosa of AR mice induced by RAGW. (A) Representative H&E staining image of nasal mucosa (left) and quantification of nasal mucosal thickness (right). Magnification = 20 ×, scale bar = 100 μm; n = 5. (B) Representative chromogenic acid staining image (left) and eosinophil counts (right) of nasal mucosa. Magnification = 40 ×, scale bar = 50 μm; n = 5. (C) Representative PAS staining image (left) and goblet cell counts (right) of nasal mucosa. Magnification = 40 ×, scale bar = 50 μm; n = 5. The data are presented as the mean ± SEMs. * p < 0.05; ** p < 0.01; *** p < 0.001. RAGW, ragweed pollen; Ctrl, control; AR, allergic rhinitis; Ceti, Cetirizine; GK-L, low-dose GUANKE (10 6 CFU); GK-M, middle-dose GUANKE (10 8 CFU); GK-H, high-dose GUANKE (10 10 CFU).

Article Snippet: Cetirizine (HY-17042, MCE) was used as a positive control for this study.

Techniques: Staining, Control

Journal: Frontiers in Nutrition

Article Title: The anti-allergic effect of Lactobacillus plantarum GUANKE on allergic rhinitis induced by either ragweed pollen or house dust mites in mice

doi: 10.3389/fnut.2025.1678249

Figure Lengend Snippet: The effect of GUANKE strain on histopathological changes in nasal mucosa of AR mice induced by HDM. (A) Representative H&E staining image of nasal mucosa (left) and quantification of nasal mucosal thickness (right). Magnification = 20 ×, scale bar = 100 μm; n = 5. (B) Representative chromogenic acid staining image (left) and eosinophil counts (right) of nasal mucosa. Magnification = 20 ×, scale bar = 100 μm; n = 5. (C) Representative PAS staining image (left) and goblet cell counts (right) of nasal mucosa. Magnification = 20 ×, scale bar = 100 μm; n = 5. The data are presented as the mean ± SEMs. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. HDM, house dust mite; Ctrl, control; AR, allergic rhinitis; Ceti, Cetirizine; GK-L, low-dose GUANKE (10 6 CFU); GK-M, middle-dose GUANKE (10 8 CFU); GK-H, high-dose GUANKE (10 10 CFU).

Article Snippet: Cetirizine (HY-17042, MCE) was used as a positive control for this study.

Techniques: Staining, Control

Journal: Bioengineering (Basel, Switzerland)

Article Title: Enhancing Gas Fermentation Efficiency via Bioaugmentation with Megasphaera sueciensis and Clostridium carboxidivorans .

doi: 10.3390/bioengineering12050470

Figure Lengend Snippet: Figure 2. Course of metabolite concentration in CHER I; Indigo circles: acetate, orange squares: butyrate, green diamonds: caproate, blue triangles: ethanol; Key events: day 7—inoculation with M. sueciensis, day 49—media swap, day 119—media swap after fixing leakage; day 265—media swap. Sample for Metataxonomic analysis taken at day 315.

Article Snippet: Megasphaera sueciensis (DSMZ 17042) is a gram-negative, anaerobic, non-motile, nonspore-forming diplo-cocci strain from the Veillonellaceae family.

Techniques: Concentration Assay

Journal: Bioengineering (Basel, Switzerland)

Article Title: Enhancing Gas Fermentation Efficiency via Bioaugmentation with Megasphaera sueciensis and Clostridium carboxidivorans .

doi: 10.3390/bioengineering12050470

Figure Lengend Snippet: Figure 6 shows the composition of the consortium that was used as a starter culture for the reactors (data from Steger et al. [20]). The genus Megasphaera could not be detected in the inoculum, as demonstrated in Figure 6 and Supplementary Figure S1.

Article Snippet: Megasphaera sueciensis (DSMZ 17042) is a gram-negative, anaerobic, non-motile, nonspore-forming diplo-cocci strain from the Veillonellaceae family.

Techniques:

Journal: Bioengineering (Basel, Switzerland)

Article Title: Enhancing Gas Fermentation Efficiency via Bioaugmentation with Megasphaera sueciensis and Clostridium carboxidivorans .

doi: 10.3390/bioengineering12050470

Figure Lengend Snippet: Figure 2. Course of metabolite concentration in CHER I; Indigo circles: acetate, orange squares: butyrate, green diamonds: caproate, blue triangles: ethanol; Key events: day 7—inoculation with M. sueciensis, day 49—media swap, day 119—media swap after fixing leakage; day 265—media swap. Sample for Metataxonomic analysis taken at day 315.

Article Snippet: Microorganisms Megasphaera sueciensis (DSMZ 17042) is a gram-negative, anaerobic, non-motile, nonspore-forming diplo-cocci strain from the Veillonellaceae family.

Techniques: Concentration Assay

Journal: Bioengineering (Basel, Switzerland)

Article Title: Enhancing Gas Fermentation Efficiency via Bioaugmentation with Megasphaera sueciensis and Clostridium carboxidivorans .

doi: 10.3390/bioengineering12050470

Figure Lengend Snippet: Figure 6 shows the composition of the consortium that was used as a starter culture for the reactors (data from Steger et al. [20]). The genus Megasphaera could not be detected in the inoculum, as demonstrated in Figure 6 and Supplementary Figure S1.

Article Snippet: Microorganisms Megasphaera sueciensis (DSMZ 17042) is a gram-negative, anaerobic, non-motile, nonspore-forming diplo-cocci strain from the Veillonellaceae family.

Techniques: